HLA TYPING BY NGS
  • NGS-Based High-Resolution HLA Typing
  • Conventional methods (serology, SSP, SSO, SSB) and real-time typing offer low resolution and struggle with HLA gene complexity due to high polymorphism, inter-locus homology, and pseudogenes.
  • NGS overcomes these limitations by enabling clonal amplification and sequencing of extended regions, including introns, achieving up to 4-field (eight-digit) allele-level resolution-without added cost or effort.
  • Accelerates discovery and submission of novel HLA alleles to global databases
  • Available as CE-IVD and RUO panels for 6 loci (HLA-A, B, C, DRB1, DQB1, DPB1) and 11 loci (HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, DPB1)
  • High throughput: pool up to 384+ samples per run
  • Minimizes hybrid reads (Class I & DRB genes)
  • Reduces pseudogene amplification (HLA-H, K, DRB6, DRB8)